ABSTRACT
Objective: The present study was performed to evaluate the immunomodulatory, anticancer, and antioxidative properties of the fraction (CP_2) isolated from Cyclea peltata. Methods: Immunomodulation was evaluated in lymphocytes by lymphocyte proliferation assay and in THP-1 macrophage cell lines by MTT assay. The nitrite production by the macrophages was also measured by the nitrite assay using griess reagent. The anticancer activity of the fraction was determined by MTT assay. The antioxidant activity was evaluated by DPPH assay and total antioxidant assay by phosphomolybdenum method. It is expressed as number of gram equivalent of ascorbic acid. Results: The plant fraction showed the presence of flavonoids which induced lymphocyte proliferation rate of 4.29±0.007 at 100 μg/ml. It was not toxic to THP-1 macrophage cells and also could induce nitrite production at 1 mg/ml. It also exhibited good anticancer activity at 100 μg/ml after 48h of incubation. The DPPH activity was found to be low since 100 μg/ml showed only an inhibition rate of 22±0.026. The total antioxidant activity at 1000 μg/ml of CP_2 was found to be equivalent to 79±0.03 μg/ml of ascorbic acid exhibiting moderate antioxidant activity. Conclusion: The fraction CP_2 containing flavonoid, isolated from Cyclea peltata has good immunomodulatory, antioxidant and anticancerous property.